The RAMP Troponin I Assay

The RAMP^® Troponin I Assay is a quantitative immunochromatographic test and is used with the RAMP Reader to measure cardiac troponin I levels in EDTA whole blood.
 

Principles of the Assay

• Diluted EDTA whole blood is applied into
  the sample well of the Test Cartridge.
  
  
• The red blood cells are retained in the sample pad, and the separated plasma migrates along the strip.
  
  
• Fluorescent-dyed latex (test) particles coated with anti-TnI antibodies bind to TnI,
  if present in the sample. As the sample migrates along the strip, the TnI-latex complexes are captured at the detection zone, and excess latex particles are captured at the Internal Control Zone.
  
  
• The RAMP Reader then measures the amount of fluorescence emitted by the complexes captured at the Detection Zone and at the Internal Control Zone.
  
  
• Using a ratio between the two fluorescence values, a quantitative reading is calculated and reported.

In addition to providing precise and accurate results, this proprietary method accounts for and mitigates the effects of background signal, inherent specimen variability and interfering substances commonly found in blood utilizing the RAMP Ratio.