RAMP Ratio Improves CV

RAMP measures fluorescence emitted by complexes captured in the Detection and Internal Control Zones.
 
Data from 5 replicates of 50 ng/mL standards tested in the RAMP^® Myoglobin Assay were selected to show that even extreme differences at the Detection Zone can be corrected by use of the RAMP Internal Standard Zone.
 
Note that the replicate with higher levels of F-units at the Detection Zone also show higher levels of F-units measured at the Internal Standard Zone.
 
The RAMP Ratio calculated between these two zones corrects for test-to-test variations (CV=3.8%). Without the Ratio absolute fluorescence at the Detection Zone would be measured alone leading to extremely variable results (CV=35.1%).